All editions of Flash Media Server 3 support playback of the following file formats:
FLV
All versions of Flash Media Server (1, 2, and 3) support the FLV file format. The FLV file format supports the following codecs: On2 VP6, Sorenson Spark, and MP3.
To create FLV files from existing digital video or audio files, use third-party video encoding utilities, like On2 or Sorenson Squeeze, or export them from Flash. Recorded streams can contain data messages, as well as video and audio and are stored in the FLV file format.
F4V, MP4, M4A, MOV, MP4V, 3GP, and 3G2
Flash Media Server 3 supports playback of H.264-encoded video and AAC-encoded audio within MPEG-4 Part 14 container formats.
You can use any number of industry-standard tools to create files in these formats with these types of encoding, including Adobe Premiere and Adobe AfterEffects.